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KMID : 0613820220320010001
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Journal of Life Science
2022 Volume.32 No. 1 p.1 ~ p.10
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Characterization of Antidiabetic Compounds from Extract of Torreya nucifera
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Kim Ji-Won
Kim Dong-Seob
Lee Hwa-Sin
Park Bo-Bae
Yu Sun-Nyoung
Hwang You-Lim
Kim Sang-Hun
Ahn Soon-Cheol
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Abstract
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Natural products have gained increasing attention due to their advantage of long-term safety and low toxicity for a very long time. Torreya nucifera is widespread in southern Korea and Jeju Island and its seeds are commonly used as edible food. Oriental ingredients have often been reported for their insecticidal, antioxidant and antibacterial properties, but there have not yet been any studies on their antidiabetic effect. In this study, we investigated several biological activities of T. nucifera pericarp (TNP) and seeds (TNS) extracts and proceeded to characterize the antidiabetic compounds of TNS. The initial results suggested that TNS extract at 15 and 10 μg/ml concentration has inhibitory effects on α-glucosidase and protein tyrosine phosphatase 1B, that is 14.5 and 4.35 times higher than TNP, respectively. Thus, the stronger antidiabetic TNS was selected for the subsequent experiments to characterize its active compounds. Ultrafiltration was used to determine the apparent molecular weight of the active compounds, showing 300 kDa or more. Finally the mixture was then partially purified using Diaion HP-20 column chromatography by eluting with 50~100% methanol. Therefore we concluded that the active compounds of TNS have potential as therapeutic agents in functional food or supplemental treatment to improve diabetic diseases.
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KEYWORD
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Diaion HP-20, α-glucosidase, protein tyrosine phosphatase 1B, Torreya nucifera, ultrafiltration
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